BL-IH Basic Langendorff Isolated Heart Systems on IH-SR base
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- Turnkey system for perfusing isolated rodent hearts in Langendorff
mode, includes perfusion system, transducers, amplifiers, data
acquisition
- Compact unit improved for low volume and high temperature
stability
- High perfusion pressure possible (up to 300 mmHg)
- Continuous measurement of heart mechanics
- Can be extended for continuous measurement bioelectrical
signals (ECG, MAP)
- Can be extended and upgraded to a full working heart system
- Applications:
– Testing inotropic and vasoactive substances
– Testing cardiac rhythm (chronotropic and arrythmogenic
effects)
– Cardiovascular screening of transgenic mice
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Specifications
Operating Modes:
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Version LH |
Only Langendorff Mode at constant pressure or constant flow |
Limiting Data:
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Aorta |
ID 0.8 to 2.5 mm |
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Perfusion Flow |
Up to 50 ml/min |
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Perfusion Pressure |
Up to 300 mmHg |
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The basic Langendorff Isolated Heart System BL-IH is a ready
to use isolated heart perfusion system for rodent hearts such
as mouse, rat and guinea pig. This system is based on our premium
IH-SR isolated heart system for constant flow or pressure perfusion.
To achieve constant pressure perfusion, the BL-IH utilizes our
SCP Controller module. The SCP module in combination with a pressure
transducer, an amplifier and peristaltic pump controls the perfusion
pressure. The complete system includes the perfusion apparatus,
the transducers and amplifiers for perfusion pressure, the servo
controller and integrated peristaltic pump, the isovolumetric
left ventricular pressure measurement (isovolumetric balloon method)
using a second pressure transducer and amplifier and a data acquisition
system. The apparatus by design is constructed to be very compact.
All of the components are mounted on a basic Plexiglas stand except
for the thermostatic circulator and the measuring instruments.
Special attention has been made to ensure constant and defined
temperature conditions at the heart. All critical parts in this
respect are arranged inside the thermostated heart chamber, thus
avoiding any cooling through a temperature gradient to ambient
temperature. Additional recording or pacing electrodes can be
arranged aroung the aortic bloc. The large thermostated space
arround the heart allows easy placement of these additional measurement
systems.
The connection to the heart is made through interchangeable aortic
cannulae. The perfusate is aerated by bubbling in the reservoir.
When using perfusates tending to foam or with certain additives
it is possible to use an optional membrane oxygenator. The BL-IH
is optimized and intended only for Langendorff heart perfusion.
The system is simple to setup and use and is available for mouse,
rat, and/or guinea pig hearts. The use of the SCP module allows
continuous recording of flow rate without an expensive flowmeter.
The following signals are recorded:
- Isovolumetric left ventricular pressure (Balloon)
- dLVP/dt
- Perfusion pressure
- Perfusion flow
The software evaluates the following parameters:
- Systolic and diastolic LVP
- Heart rate
- dP/dt max, dP/dt min, Contractility index
- Mean perfusion pressure
- Mean perfusion flow
- Coronary resistance
HUGO SACHS ELEKTRONIK-HARVARD
APPARATUS GmbH
Gruenstrasse 1 · D-79232 March-Hugstetten · GERMANY
Phone: +49 (0) 76 65 - 92 00-0 · Fax: +49 (0) 76 65 - 92
00-90
