Isolated Perfused Lung for Rat/Guinea Pig Size 2, IL-2
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- Optimized in-situ preparation
- Negative-pressure ventilation to mimic more closely the in-vivo
situation
- Regular induction of hyperinflation of the lung (sigh, augmented
breath)
- Several perfusion modes using buffer solution or blood are
available:
– Constant flow perfusion
– Constant pressure perfusion
– Dual perfusion system (constant flow or constant pressure
can be selected)
- Continuous measurement of lung mechanics (tidal volume, resistance,
compliance), and perfusate characteristics (pressure, flow, pO2,
pCO2, pH)
- Continuous measurement of lung weight (Edema)
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Ventilation
The lung is placed in the artificial thoracic chamber and ventilated
at negative pressure, it is connected over the tracheal cannula
to the pneumotachometer for measurement of respiratory flow. The
humidified ventilation medium (air or gas mixture) is constantly
flowing past the distal end of the pneumotachometer.
Perfusion
Lungs can be perfused by either constant flow or constant pressure.
Although constant flow perfusion may mimic the in vivo situation
more closely, it has the disadvantage that hydrostatic edema becomes
inevitable during vasoconstriction. Constant pressure perfusion
permits higher perfusate flow rates since vasoconstriction decreases
perfusate flow and hydrostatic edema is less likely to occur.
Can be configured in one of three different perfusion modes:
1. Constant Flow Perfusion
2. Constant Pressure Perfusion
3. Dual System to Switch from Constant Flow to Constant Pressure
and Vice Versa
Lung Weight Changes
Continuous measurement of weight changes of the lung is required
for monitoring edema formation. This is possible during negative
pressure ventilation inside the thoracic chamber by using the
HSE-Edema balance LS 30 (weight transducer) (NP 73-0593), range
±30 g, displacement 2 µm/gram. Weight changes induced
by raising or lowering the perfusion pressure can be used to assess
vascular permeability by determining the filtration coefficient.
Checklist for more information
or quotation on
Isolated Lung Systems
HUGO SACHS ELEKTRONIK-HARVARD
APPARATUS GmbH
Gruenstrasse 1 · D-79232 March-Hugstetten · GERMANY
Phone: +49 (0) 76 65 - 92 00-0 · Fax: +49 (0) 76 65 - 92
00-90