- Basic test in gastrointestinal research
- Peristaltic reflex in guinea pig ileum
- For studying emptying phase of peristaltic reflex
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This tissue bath has been designed for studying the peristaltic
reflex in the guinea pig
ileum. The circular muscle contraction which propels the contents
of the ileum from aboral to
anal direction is induced by the increase of the intraluminal
pressure up to a threshold point. The tissue bath is a water-jacketed
Plexiglas bath. The holder for the cannulae can be removed from
the main bath during the preparation. A 4 to 5 cm long segment
of ileum, where the mesenteric has been removed and which has
been flushed is cannulated in the oral and aboral ends and tided.
Three different sizes of cannulae are available. The cannulae
are fixed on sliding holders to optimize the distance between
the cannulae and adapt to the individual length of the ileum avoiding
any slackness of the tissue. The inflow is controlled by means
of a hose pump with a flowrate of about 0.5 ml/min. The direction
of the perfusion flow must meet the physiological way. The outflow
can be switched using a stopcock from a level equivalent to the
level of the bath to a level of about 3 cm above the level of
the fluid in the bath. The peristaltic reflex is induced by changing
the position of the outflow stopcock. When it is in position ‘peristalsis’,
the pressure increases slowly resulting in a distention of the
ileum until the pressure threshold level is reached. At this time
the emptying phase is started as a visible contraction of the
circular muscle moving from the oral to the aboral side. The intraluminal
pressure is recorded from the oral side by a pressure transducer
and the appropriated amplifier and recorder. The pressure transducer
must be sensitive enough to reproduce the small threshold point
of 1 to 2 cmH2O
HUGO SACHS ELEKTRONIK-HARVARD
APPARATUS GmbH
Gruenstrasse 1 · D-79232 March-Hugstetten · GERMANY
Phone: +49 (0) 76 65 - 92 00-0 · Fax: +49 (0) 76 65 - 92
00-90