- Perfusion of tubular organs (trachea, blood vessels, intestines)
- Individual solutions for intraluminal perfusions and extraliminal superfusion
- Controlled perfusion pressure
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This Tissue Bath has been designed for the studying of perfused
tubular organs such as trachea, blood vessels , intestines and
vas deferens. Individual solutions can be used for intraluminal
perfusion and extraliminal superfusion. Two roller pumps are needed,
one for intraluminal constant flow perfusion and the second for
extraliminal superfusion. The effective intraluminal perfusion
is generated by an adjustable afterload control system. A differential
pressure transducer is used to measure the intraluminal pressure
difference at the proximal and distal end of the organ. The tissue
bath is a water-jacketed Plexiglas bath. The holder for the cannulae
can be removed from the main bath during the cannulation of the
segment of tubular organs. Four different sizes of cannulae are
available. The cannulae are fixed on sliding holders to optimize
the distance between the cannulae to adapt to the individual length
of the organ avoiding any slackness of the tissue. There are two
afterload control systems available. One for low pressure
applications (0 to 20 mmHg) and the second for high pressure applications
(10 to 300 mmHg). The unit is supplied with four different interchangeable
adapters for use with vessels or organs with the following diameters:
1.5, 2.5 , 3 and 4 mm. For smaller vessels customized stainless
steel cannulae can be made on request. For microvessels, glass
capillary pipettes pulled to the required diameter can be connected
to the adapter cannulae using a silicone tube collar.
HUGO SACHS ELEKTRONIK-HARVARD
APPARATUS GmbH
Gruenstrasse 1 · D-79232 March-Hugstetten · GERMANY
Phone: +49 (0) 76 65 - 92 00-0 · Fax: +49 (0) 76 65 - 92
00-90