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Perfusion System for Isolated Pig Liver or Pig Kidney

For use in physiological or pharmacological research for the perfusion of a pig liver or kidney with blood or erythrocyte containing perfusate

Basic System

The pig liver to be perfused is placed in a moist, thermostated chamber and perfused with blood through the portal vein under constant-flow conditions. A centrifugal pump with a gentle action on blood is employed to reduce hemolysis; it is controlled by an electronic controller (SCP) so that the apparatus can be operated under constant-flow conditions. The system can also operate under constant-pressure conditions. Flow control requires that the flow is measured by means of a flow probe (16N) and a flowmeter (TTFM). The pressure should also be measured to monitor the experiment. This requires a pressure transducer (P75) and a bridge amplifier (TAM). A suitable oxygenator is employed for oxygenating and warming the blood; it can be of a type used in human medicine, e.g. Terumo Capiox SX10®.

Components required

The following items are required for operating the apparatus:

• Thermostated moist chamber Type 869/2
• Circulating thermostat of approx. 3 liter capacity, distilled water and an anti-algal agent, e.g. Thermoklar
• Pump with electrical control. For blood we recommend a Pump Drive BVP-ZX with centrifugal pump head
• Servo Controller for Perfusion (SCP) Type 704
• Flowmeter (TTFM) with flow probe (16N)
• Recommended: pressure transducer (P75) and bridge amplifier (TAM-D)
• Oxygenator with heat exchanger, e.g. Terumo Capiox SX10®
• Source of Carbogen for aeration. Central supply or Carbogen cylinder with pressure reducing valve

Additional monitoring equipment can be added to the system as required to create a custom, application specific, turn key system for your unique research needs.

Checklist for more information
or quotation on
Isolated Organ Perfusion Systems

HUGO SACHS ELEKTRONIK-HARVARD APPARATUS GmbH
Gruenstrasse 1 · D-79232 March-Hugstetten · GERMANY
Phone: +49 (0) 76 65 - 92 00-0 · Fax: +49 (0) 76 65 - 92 00-90