Perfusion System for Cell Isolation
PSCI
PSCI with operating table for in-situ perfusion
PSCI with Heart extension
- For cell isolation from mouse, rat, guinea pig organs by
disintegration
using colagen (protease) - Dual perfusion system for blood cells flush out and colagen disintegration
- Dedicated extension for cardiomyocites isolation
Heat Exchanger Inside
Diameter: 1.5 mm (0.06 in) for M Version
2.0 mm (0.08 in) for R Version
Maximum Flowrate: 50 m/min for the M Version
100 ml/min for the R Version
Prime Volume: < 3ml for the M Version
< 5ml for the R Version
Dimensions W x D x H: 600 x 400 x 570 mm (23.6 x 15.8 x 22.5 in)
Weight: 8 kg (17,6 lbs)
The apparatus PSCI is specially designed for harvesting individual
cells from isolated organs like mouse, rat or guinea-pig hearts,
liver... It is feasible that the apparatus can also be used for
disintegrating any perfused organ. Individual cells are released
from the cell structure of the tissue through perfusion with
protease solution and are then flushed out.
The apparatus has two separate perfusion circuits. The organ
can be switched between the two circuits by means of a changeover
stopcock. One circuit is filled with conventional perfusion solution
(e.g. Krebs-Henseleit solution, KHS) and is used in the initial
phase to flush out all blood cells from the organ. During the
second phase the apparatus is switched to the second circuit
which operates with protease solution and where disintegration
takes place.
In case of using a heart, atmospheric bacteria are kept away
from the heart through suitable construction of the apparatus
and by creating a small positive Carbogen pressure. Throughout
the entire perfusion process the heart is contained within a
system closed to the outside
atmosphere. It is only opened after the termination of perfusion
in order to remove the heart.
Perfusion takes place under constant-flow conditions. The limits
of the apparatus are a flow rate of about 50 or 100 ml/min depending
on the configuration. The apparatus is so designed that the individual
steps required for preparing the cells can proceed as simply
and clearly as possible. The components wetted by the perfusion
solutions are made from materials resistant to alcohol so that
the apparatus can be filled with alcohol for sterilisation.
The suitable constant flow is adjusted on the peristaltic pump.
The dual heat exchanger is mounted on a vertical panel which
in turn is secured to a rigid main vertical stainless steel column.
A platform for the roller pump is also secured to the vertical
column. The remaining components are not fixed to the basic setup
but are positioned
on the baseplate and are suitably connected by tubing. All components
secured to the vertical steel column can be moved to suitable
operating positions after releasing clamping screws. The protease
reservoir and the holder for the perfusion pressure transducer
are also part of the main system.
The main system is universal and must be adapted to the
organ used by adding options.
The following options are available:
The dual heat exchanger:
-Stainless steel tube ID = 1.5 mm,Volume approx. 1ml for the
PSCI-M
-Stainless steel tube ID = 2 mm ,Volume approx. 1.5 ml for the
PSCI-R
The heat exchanger steel tubing can be interchanged.
Reservoir for flushing solution:
0.5, 1, 2 L depending on the organ used and the amount of perfusate
required
Adaptation for Heart perfusion:
In case of using the heart version, a jacketed heart chamber
is mounted on a slide which is clamped to the vertical column.
A slow gas flow into this chamber does ensure a positive pressure
inside the chamber at all times, thus preventing ingress of bacteria
from the surroundings during operation.
Adaptation for in-situ perfusion (e.g. liver):
by adding an operating table and the relevant cannulae depending
on the organ
Adaptation for in-vitro perfusion:
by adding a jacketed moist chamber and the relevant cannulae
depending on the organ