Checklist to quote an Isolated Heart System
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Species

Mice   Rats   Guinea-pig    Rabbit    Small pig     

If others please specify:  

Perfusion mode

Retrograde perfusion according to Langendorff
      Langendorff only
      Later upgrade to a Working Heart system  possible

 Ejecting, Working Heart according to Neely

Perfusion solutions:

 Krebs-Henseleit Solution     Eryhtrocyte containing solution    Blood    

If others please specify:  

Measurement System:

Langendorff System

 Left Ventricular Pressure LVP with balloon method
 Perfusion Pressure
 Coronary Flow

Working heart

 Preload Pressure
 Aortic Pressure
 Atrium Flow (Cardiac Output)
 Aortic Flow
 LVP with TIP catheter
 LV pressure volume loop
 Coronary Flow

Electrophysiology:

 Single Lead ECG
 Single epicardial Monophasic Action Potential MAP
 Single endocardial Monophasic Action Potential MAP

Multi-lead ECG and multiple epicardial MAP (only on rat and larger hearts)
 6 - lead ECG
        (I, II, III, aVR, aVL, aVF)
 12 - lead ECG
        (I, II, III, aVR, aVL, aVF, V1 - V6)
 multi-epicardial MAP
          2 3 4 5 6 7 8 Number of MAP

Heart Mechanics: (only on larger hearts)

 Segment length (Sonomicrometry)
 Wall thickness (Sonomicrometry)
 Wall thickening (Sonomicrometry)

Others

 Temperature
Gas concentrations: (only on larger hearts)
 pO2
 pH

Electrical Stimulation:

 Pacing

Data acquisition:

 Computer data acquisition
 Desktop Computer    laptop
 PC-Computer available

For evaluation of typical parameters, such as:

• From LVP Signal: LVPsys, LVPdia, LVPEDP, dP/dtmin, dP/dtmax, Heart Rate, Mean Pressure
  
• From All Pressures: Systolic, Diastolic and Mean Pressure, Heart Rate
• From All Flows: Mean, Max. and Min. Flow
• From Distance: Max., Min. and Amplitude (Max-Min)
• From Electrogram: RR-I, Heart Rate
• From temperature, pO2, pH Signals: Mean Value
• It is also possible to calculate specific values from these parameters
  by writing a formula (e.g., the ratio of dP/dtmin / dP/dtmax or LVPEDP / heart weight, etc.).
  

Additional Software Modules:

• Module LVP Advanced:
  Tau, Time to Peak (Contractility Time), Relaxation Time and Contractility Index CI = dP/dtmax / P, Ejection Time
  
• Module Flow Advanced:
  Endsystolic Flow ESFL, Enddiastolic Flow EDFL
• MAP module:
  Max, Min, Plateau, Amp, Rate, ±dv/dt, duration at 10% of monophasic action potentials
• Dimension module:
  end-systolic length ESL, end-diastolic length EDL, +dL/dt, -dL/dt