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Simple Test on Anesthetized Spontaneously Breathing Rodents

For measurement of bronchospasmolysis in anesthetized spontaneously breathing rodents (Rat, Guine-Pig, Mouse) Basic simple system Evaluation of typical parameters for respiratory studies (flow, tidal volume, minute volume, respiratory rate, inspiratory and expiratory duration For acute experiments or intubated animals

The rodent is connected to a HSE-PTM or Fleisch pneumotachometer of suitable size through a tracheal or an intubation cannula.When using a Fleisch tube, a special adpater allows the connection of a trachea or intubation canula with a minimum of dead space. A heating transformer for the Fleisch tube is also available to prevent the formation of condensate. The HSE-PTM has especialy been designed to present low dead space and is made of Plexiglas to prevent condensate. The pressure difference produced at the pneumotachometer is converted by a differential pressure transducer VALIDYNE DP 45-14 into an electrical signal proportional to the respiratory flow. The tubing must be kept short to prevent the signal from being distorted by a large dead space between pneumotachometer and differential pressure transducer. A single or multi channel system can be setup using a PLUGSYS system. A pneumotachometer, a differential pressure transducer and a CFBA amplifier are required tor each animal. The HSE- PULMODYN Software is used for data acquisition and evaluation.

The following parameter are possible:

• Maximal inspiratory and expiratory air flow
• Tidal volume and minute volume
• Respiration rate
• Duration of Inspiration and duration of Expiration
• Apnea Time
• Time of brake and Time of pause
• Airflow at 0.5TV

HUGO SACHS ELEKTRONIK-HARVARD APPARATUS GmbH
Gruenstrasse 1 · D-79232 March-Hugstetten · GERMANY
Phone: +49 (0) 76 65 - 92 00-0 · Fax: +49 (0) 76 65 - 92 00-90