- Easy to use
- Constant voltage monophasic, biphasic or alternating stimulation (> 1A)
- High output impulse current
- Backup memory for timing setting
- Specially adapted for isolated cells stimulation
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The stimulator CS Type 223 is a new development of an electronic
stimulator for the electrical stimulation of isolated cells, e.g.
myocytes. Stimulating isolated cells needs a high impulse current
to trigger a maximum of cells independent of the size, shape specially
in cell culture medium having a low resistance. This is of importance
in experiment where the threshold voltage of cell reaction has
to be determined. To reduce electrochemistry effects such as polarization
of the electrodes and double layer of gas bubbles biphasic stimulation
with perfect symmetry is requested. To be able to activate an
action potential by depolarizing the membrane and to avoid further
influence of the stimuli on the cell reaction, short and powerful
impulses are required.
Stimulation Mode and Timing are displayed on a LCD display and
can easily be set in a step through mode using the ‘Menu’
button and the adjust knob. The last setting of the timing is
stored in a backup memory and recalled automatically after switching
on the instrument. The Stimulus Output Voltage is indicated on
the built-in digital voltmeter and can be Adjusted on two potentiometers:
“coarse” and “fine” with adjustment in the
range of 0 to 45 V. The output is floating, not connected to earth
(ungrounded). The output has a very low impedance (less than 1
Ohm). To avoid overheating of the output circuit a dynamic current
limiter is built in. It limits the maximum output current relative
to the Stimulation Width.
| Stimulation Mode |
SINGLE/STRT single stimulus triggered by START switch or by remote contact REPEAT/STRT repeating stimulus triggered by START switch or by remote contact TRIGGER pulse (TTL, negative-going edge) starts delay circuit; START switch must be in OFF position |
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Stimulation Frequency |
0.05 to 100 Hz (in 29 steps) 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 Hz |
| Delay Time |
(leading the stimuli): 2 to 50 msec (in 7 steps) 2, 5, 10, 20,
30, 40, 50 msec |
| Stimulus Width |
10 µsec to 10 msec (in 28 steps) 10, 20, 30, 40, 50, 60,
70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 µsec 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 msec |
| Stimulus Output |
1. Output voltage 0 to 50 V; Pulse Current up to 1 A at max. pulse width (10 msec) 2. Monophasic, Biphasic, Alternated 3. Output circuit floating, not connected to earth (ungrounded) |
| Indication |
1. LCD module (4 lines of 16 characters each) to indicate stimulus parameter settings (except amplitude); 2. Digital voltmeter (DVM) to indicate output voltage setting range selectable: 0 - 19.99 V <—> 0 - 50.0 V 3. LED for stimulus pulse 4. Beep (with each stimulus pulse), can be switched off 5. 2 LEDs for polarity of next stimulus: + or - |
| Trigger Input |
BNC socket, input signal starts time delay, TTL level, negative active edge |
| Trigger Output | BNC socket, signal: delay time, open collector output (2 k. to +5V) |
| Weight | 3.5 kg (7.7 lb) |
| Dimensions, H x W x D | 110 x 300 x 250 mm (4.3 x 11.8 x 9.8 in) |
HUGO SACHS ELEKTRONIK-HARVARD
APPARATUS GmbH
Gruenstrasse 1 · D-79232 March-Hugstetten · GERMANY
Phone: +49 (0) 76 65 - 92 00-0 · Fax: +49 (0) 76 65 - 92
00-90