Checklist to quote an Isolated Tissue System
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Tissues and Species

Strips     Rings     Vessels

Please specify type of tissue:    Aorta, Vene, Trachea, Ileum, Skelettal muscle, Papillary muscle, Left Atrium, Right atrium, Urether...

 Number of tissues in parallel

from

Mice     Rats     Guinea-pig     Rabbit      Pig     

If others please specify species:  

Mode

Incubation / Immersion
 Vertical tissue bath (Graz , Schuler)
 Horizontal tissue bath (Mayflower)

Superfusion mode
 Single tissue vertical (Coleman)
 Single tissue horizontal (Mayflower)
 Cascade mode on vertical tissues (Schuler Cascade)

 Electrophysiology, Intracellular Action Potential (Steiert bath)

Solution used:

 Krebs-Henseleit Solution      Ringer solution     Tyrode    

If others please specify:  

Measurement System:

 Isometric contractile force
 Isotonic displacement
 Auxotonic contraction

 Intracellular Action Potential (only if Mode Electrophysiology)

Electrical Stimulation:

 Field Stimulation
 Direct Stimulation

Data acquisition:

 Computer data acquisition
 Desktop Computer     laptop
 PC-Computer available

Parameters to evaluate:

 Resting Tension

The following parameters require a beating or an electrical stimulated tissue:
 Beating Rate
 Developped Force
 Maximum Force
 Contraction time
 Relaxation time
 Response duration
 Peak duration
 Latency periode
 +/- dF/dt

 Action Potential Evaluation:
       - CT (conduction Time)
       - TOP (Take Off Potential)
       - AMP (Amplitude), dV/dt (Max. rate of depolarisation)
       - EOP (Potential after end of AP)
       - APD90 APD75 APD50 APD25 APD10 (Siganl duration at 90, 75, 50, 25, 10%)