IPL-16 Core Isolated Perfused Lung System for Pig and Other Large Species

The IPL-16 system is designed for whole lung or single lung lobe perfusion of pig or species lung of similar size.

The IPL-16 core system contains all the primary equipment to accomplish basic perfusion and ventilation experiments, requiring only the addition of ventilation equipment. The basic system can be easily upgraded with options such as aerosol delivery, real-time flow measurement and many others.

Advanced System Design
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Standard Options
Ventilation
Deoxygenation
Specialized Applications & Options

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 Advanced System Design

The lung chamber and lid are both water jacketed and when connected with a heating or heating/coiling thermocirculator ensure accurate maintenance of organ temperature. A traditional heating thermocirculator is typically used with studies that are physiologically isothermic and heating/coiling thermocirculators with integrated chillers are used for studies replicating organ transplantation conditions. The unique cannulation system allows the researcher to remove the cannulation block and the lung platform to cannulate the vasculature and trachea outside of the main chamber while maintaining physiological pulmonary artery pressure and continuous ventilation.

The lung in this system can be only positive-pressure ventilated. A clinical ventilator or our Model 613 Large Animal Ventilator can be used. The space below the lung platform is used as integrated reservoir designed for recirculating perfusate. A single lobe or the full lung can be perfused through one or more pulmonary arteries. The base lung chamber has an integrated lung weight sensor which when connected to the required TAM-A amplifier provides continuous monitoring of lung weight and tracking of edema formation.

When the lung is placed in the chamber and ventilated a pneumotachometer for measurement of respiratory airflow is connected. PULMODYN software can calculate the tidal volume and other respiration parameters from the airflow. Ponemah Data Acquisition & Analysis Software from DSI is also suitable.

Constant Flow or Constant Pressure Perfusion

The lung can be perfused at a constant flow rate or under constant pressure.The SCP module controls and regulates the perfusion pressure in the pulmonary artery. Pump selection is often based on the composition of the perfusate. For acellular media, a peristaltic pump can be used. If blood or blood-doped perfusion solutions are to be used, a centrifugal pump can be used.

For acellular media and a configuration including a peristaltic pump, the system is typically configured with a pressure transducer, amplifier and the PLUGSYS SCP module that receives the pressure signal from the amplifier and controls the pump speed. A switch on the SCP allows the user to change between either constant flow or constant pressure perfusion. Flow rate based on tubing ID and pump speed can be calibrated and recorded from the SCP module.

For cell-based media and use of a centrifugal pump, the user must specify a setup for either constant pressure or constant flow perfusion. Constant pressure perfusion is achieved as described above when a centrifugal pump is selected. Constant flow perfusion with a centrifugal pump is also possible by selecting a suitable in-line flow probe and flow meter which provides a precise flow feedback signal to the SCP for constant flow perfusion.

Lung Mechanics

The tracheal pressure is measured with an MPX pressure transducer. Airflow is determined with a hearted pneumotachometer connected to a DLP2.5 differential pressure transducer. PULMODYN Data Acquisition Software (or other suitable DAQ system such as DSI Ponemah) acquires the raw airflow and airway pressure signals and derives a variety of respiratory parameters such as tidal volume, respiratory rate and other values for respiratory mechanics from these.

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Features & Benefits

• Only Positive Pressure Ventilation (a ventilator for human use must be used)
• Optimized temperature conditions for the isolated lung
• Unique jacketed chamber
• Continuous measurement of lung weight to monitor edema formation
• Drug injection pathway built directly into pulmonary perfusate stream for precision compound dosing
• Optimized preparation
• Perfusion under constant flow or constant pressure condition using buffer solution, heparanized blood or erythrocyte-containing solutions
• Continuous measurement of respiratory mechanics (respiration rate, inspiratory and expiratory air flow, tidal volume, minute volume, dynamic airway resistance, dynamic compliance) and perfusion characteristics (pulmonary artery pressure, left atrium pressure, lung vascular resistance, pO₂, pCO₂, pH)
• More measurement parameters with greater precision than any other system

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Applications

• Investigation of ventilation and perfusion in the isolated pig lung
• Drug testing on respiration and vascular parameters
• Aerosol tests, surfactant studies, etc.
• Continuous measurement of lung weight changes (edema studies)

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Measured Signals and Calculated Parameters

Signals recorded as raw data:

• Respiratory airflow
• Tracheal pressure
• Pulmonary artery (perfusion) pressure
• Perfusion flow

Parameters calculated from raw data*:

• Respiration Rate
• Peak inspiratory and expiratory airflow
• Tidal volume, minute volume
• Vascular resistance
• End-inspiratry and end-expiratory pressures
• Dynamic airway resistance and compliance
• Inspiratory time and expiratory time (software option)

* Calculations are automatic when PULMODYN data acquisition software is used.

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Included Items

Included items are representative of a typical IPL-16 Core System. Individual components can be customized to your needs.

IPL-16 Core System,  230 V (73-4301) includes:		IPL-16 Core System, 115 V (73-4302) includes:
Item #	Description	Item #	Description
73-4726	Base Unit for the Pig Isolated Perfused Lung	73-4726	Base Unit for the Pig Isolated Perfused Lung
73-0125	Lauda Thermostatic Circulator, Type E-103, 230 V/50 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C	73-2802	Lauda Thermostatic Circulator, Type E-103, 115 V/60 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C
73-0045	PLUGSYS Case, Type 603*	73-0045	PLUGSYS Case, Type 603*
73-2806	Servo Controller for Perfusion (SCP)	73-2806	Servo Controller for Perfusion (SCP)
Perfusion Pressure Measurements
73-0020	Low Range Blood Pressure Transducer P75 for PLUGSYS Module	73-0020	Low Range Blood Pressure Transducer P75 for PLUGSYS Module
73-1793	PLUGSYS Transducer Amplifier Module (TAM-D)	73-1793	PLUGSYS Transducer Amplifier Module (TAM-D)
Respiratory Flow Measurements
72-6310	Heated Linear Pneumotachometer with Luer side port for airway pressure,0 to 160 L/min flow rate, deadspace 13.87 ml. Port OD = 22 mm, ID = 15 mm. Opposite port: OD =15 mm, ID = 13.2 mm. Requires heater controller.	72-6310	Heated Linear Pneumotachometer with Luer side port for airway pressure, 0 to 160 L/min flow rate, deadspace 13.87 ml. Port OD = 22 mm, ID = 15 mm. Opposite port: OD =15 mm, ID = 13.2 mm. Requires heater controller.
59-9703	Heater Controller for Single Pneumotachometer 230 VAC, 50 Hz	59-9702	Heater Controller for Single Pneumotachometer 115 VAC, 60 Hz
73-3882	Differential Low Pressure Transducer DLP2.5, Range ± 2.5 cmH2O, HSE Connector	73-3882	Differential Low Pressure Transducer DLP2.5, Range ± 2.5 cmH2O, HSE Connector
73-0065	PLUGSYS Transducer Amplifier Module (TAM-A)	73-0065	PLUGSYS Transducer Amplifier Module (TAM-A)
Artificial Thorax Pressure
73-0064	Differential Pressure Transducer MPX, Range ± 100 cmH2O, HSE Connector	73-0064	Differential Pressure Transducer MPX, Range ± 100 cmH2O, HSE Connector
73-0065	PLUGSYS Transducer Amplifier Module (TAM-A)	73-0065	PLUGSYS Transducer Amplifier Module (TAM-A)

* If your chosen options require more than 20 slot units you will need to add a case extension. 

The Base Unit (73-2266) includes: 
Chamber consisting of a jacketed Plexiglass box and a jacketed cover. The chamber itself acts as the reservoir. The box is divided in two compartments by a platform receiving the organ. The platform is attached to the weighing system. The lower compartment is the perfusate reservoir. The chamber has connections for ventilation and perfusion. Perfusion cannula depend on application and must be purchased separately. Inside dimensions: 400 x 400 x 155 mm. Height for organ between platform and cover: 123 mm. Outer dimensions of chamber: 590 x 590 x 232 mm. Note: A 6 L jacketed reservoir can be added in case the perfusate cannot be recirculated.

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Standard Options (Purchase Separately)

Cannulae

Although cannulae are included with other IPL systems, they are not included with the IPL-16. Cannula are chosen by the researcher.

If a lung lobe is perfused standard Luer cannulae can be used:

• Tracheal Cannula for Rabbit/Cat, OD 6.0, L 40 mm (73-3319)
• Tracheal Cannula for Rabbit, OD 5.0, L 35 mm (73-0037)
• Tracheal Cannula for Rat/Guinea Pig/Rabbit, OD 4.0, L 32 mm (73-0036)
• Aortic Metal Cannula with Luer, OD 4.0 mm (73-2872)

If a complete lung is being perfused, larger Tygon tubing canbe used directly as cannulae.

Pumps

Either a peristaltic or centrifugal pump can be used, depending on the perfusate used.

Peristaltic Pump

The Harvard Apparatus Peristaltic Pump consists of a control unit, a motor drive, a tubing cassette and some sample tubing. The pump can deliver solutions over a range of flow rates from 0.001 to 230 ml/min. Tracheal pressure can be doubled by using two tubes in parallel.

• Complete with Control Box and P-230 Motor Drive (70-7001)

Centrifugal Pump

The Centrifugal Pump is specifically designed for pumping blood and/or erythrocyte suspension solutions. It consists of the pump drive BVP-ZX and a centrifugal pump head which can be replaced without tools.

• Centrifugal Pump Drive (BVP-ZX), 230 VAC (73-2470)
• Centrifugal Blood Pump Head (BP-80) (73-2807)

PULMODYN Data Acquisition Software

Online evaluation of a wide range of signals and classical respiration parameters.

Note: Ponemah Data Acquisition & Analysis Software from DSI, a Harvard Bioscience Company is also suitable.

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Ventilation

The Large Animal Volume-Controlled Ventilator, Model 613 is suitable for ventilating a pig lung lobe. An alternative to the 613 ventilator is a suitable clinical ventilator.

The Model 613 has adjustable tidal volume from 30 to 700 ml/stroke and an adjustable respiratory rate from 7 to 50 breaths/minute. 

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Deoxygenation

Hugo Sachs does not supply the oxygenator/deoxygenator for the IPL-16 system. Researchers can use readily available oxygenators used in the heart-lung machines of their affiliated hospital or institute. We offer two custom oxygenator holders (platforms) for use with the IPL-16 system. The platform receives the mounting parts for either the Medtroni or Terumo oxygenator. It is equipped with a rotameter for controlling the gas flow through the oxygenator.

• Base Platform for Metronic Carmeda Affinity NT Oxygenator (73-4313)
• Base Platform for Terumo Capiox CX 210 Oxygenator (73-2805)

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 Specialized Applications & Options

Real-Time Flow Measurement

Direct real-time flow measurement with functional IPL systems. 

Venous Pressure Measurement

Venous pressure transducer and amplifier for venous pressure measurement in an isolated perfused lung system.

Temperature Measurement

Measure perfusate temperature in any isolated perfused organ system.

Perfusion Solution Monitoring

Permits precise continuous or discontinuous measurement in liquid media or perfusate of these three key parameters: pO₂, pH and pCO₂.

Perfusate Filtration

For filtration of recirculated perfusate.

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