Cardiovascular Waveform Pump
The Cardiovascular Waveform Pump (former name Pulsatile Blood Pump) simulates the ventricular hemodynamic waveform of the heart. It features silicone rubber-covered heart-type ball valves and smooth flow. Only inert materials like silicone rubber, acrylate, and PTFE contact the fluid. The pumping head is easy to take apart and reassemble and can be sterilized. It can be used for circulating emulsions, suspensions, and non-Newtonian fluids such as blood. Actuation of the ball check valves will result in some hemolysis over time when using blood or blood cell doped solutions but acute use with these solutions in mock circulatory loops can be performed. Four different models are available with minute volumes of 1 - 200 ml, 10 ml – 2 L, 80 ml – 6 L, 150 ml – 10 L. It is ideal for testing flow circuits and medical devices that require ventricular hemodynamic flow morphology.
Tubing listed in the table below for connection to inlet and outlet pump ports. Other tubing formulations and sizes as well as connector kits are available.
We offer turnkey solutions to measure pressure and flow throughout your perfusion circuit. Please contact us for assistance configuring a setup that meets your experimental needs: email us at sales@harvardapparatus.com or call us at 800-597-0580. In Europe, please call +49 7665 92000 or email sales@hugo-sachs.de.
The Cardiovascular Waveform Pump (former name Pulsatile Blood Pump) simulates the ventricular hemodynamic waveform of the heart. It features silicone rubber-covered heart-type ball valves and smooth flow. Only inert materials like silicone rubber, acrylate, and PTFE contact the fluid. The pumping head is easy to take apart and reassemble and can be sterilized. It can be used for circulating emulsions, suspensions, and non-Newtonian fluids such as blood. Actuation of the ball check valves will result in some hemolysis over time when using blood or blood cell doped solutions but acute use with these solutions in mock circulatory loops can be performed. Four different models are available with minute volumes of 1 - 200 ml, 10 ml – 2 L, 80 ml – 6 L, 150 ml – 10 L. It is ideal for testing flow circuits and medical devices that require ventricular hemodynamic flow morphology.
Features
- Reproduction of the ventricular hemodynamic waveform including temporal phasing of systole (dispensing) and diastole (refilling).
- Durable construction for hours of continuous operation.
- 4 flow rate range models, Minute volumes:
- 1 - 200 ml, 10 ml – 2 L, 80 ml – 6 L, 150 ml – 10 L
- Variable phasing for Models 1421 and 1423 (see specifications table)
- Suitable for acute studies with bloodA, ideal for simulated blood solutions, other non-Newtonian solutions as well as aqueous solutions.
A. When using blood or blood cell doped solutions hemolysis will occur over time and is affected by the mechanical closing of valves, stroke rate and potentially the fluid pathway. The user is advised to determine change in hematocrit and/or free hemoglobin at various time points during the pumping of these solutions.
Pump Mechanism
A positive piston actuator and ball check valves provide the proportioning action. The product of stroke rate times stroke volume is an accurate indicator of the flow rate. Positive piston action prevents changes in flow rates, regardless of variations in resistance or back pressure. The piston always travels to the end of the ejection stroke, independent of the volume pumped.
We offer turnkey solutions including tubing, connectors, pressure transducers, flowmeters and data acquisition platforms to complete your experimental setup.
Test Setup with Pressure and Flow Values
A simple setup is presented in the figure below with a small compliance device (Penrose tubing).
Pressure Curves
The shape of the output pressure curve is a function of both the pump action and the characteristics of the external system on the output fluid circuit. The following set of curves were obtained with Model 1421, using water as the pumped medium. In the tests, pressure transducers were inserted in three locations and continuous records obtained under varying conditions.
Varying the peripheral resistance, stroke rate, stroke volume and phase ratio allows for creation of a wide range of output flow and pressure characteristics.
- Curve A Pressure just beyond the output valve
- Curve B Pressure within the pump chamber
- Curve C Pressure just before the intake valve
Ex Vivo Organ Perfusion
An example of ex vivo organ perfusion pressure and flow values obtained from a dog lung lobe perfusion.
| Item # | 52-9552 | 55-1838 | 55-3321 | 55-3305 |
| Model No. | 1407 | 1405 | 1421 | 1423 |
| Stroke Volume Adjustable | 0.05 to 1.0 ml | 0.5 to 10 ml | 4 to 30 ml | 15 to 100 ml |
| Rate Stroke/Minute | 20 to 200 | 20 to 200 | 20 to 200 | 10 to 100 |
| Minute Volume Stroke Volume x Rate | 1 to 200 ml | 10 to 2,000 ml | 80 to 6,000 ml | 150 to 10,000 ml |
| Phasing* | Fixed Phase | Fixed Phase | Adjustable Phase | Adjustable Phase |
| 35% systole | 35% systole | 35 to 50% systole | 35 to 50% systole | |
| 65% diastole | 65% diastole | 65 to 50% diastole | 65 to 50% diastole | |
| Port ID | 0.25” (6.35mm) | 0.25” (6.35mm) | 0.375” (9.52mm) | 0.5” (12.7mm) |
| Port OD | 0.325” (8.26mm) | 0.325” (8.26mm) | 0.44” (11.18 mm) | 0.56” (14.22mm) |
| Tube ID | 8 mm (0.31 in) | 8 mm (0.31 in) | 11 mm (0.437 in) | 14 mm (0.551 in) |
| Dimensions H x W x D | 312 x 156 x 250 mm (12.3 x 6.1 x 9.9 in) |
312 x 156 x 250 mm (12.3 x 6.1 x 9.9 in) |
500 x 212 x 337 mm (19.7 x 8.4 x 13.4 in) |
500 x 212 x 337 mm (19.7 x 8.4 x 13.4 in) |
| Weight | 7.3 kg (16 lb) | 7.3 kg (16 lb) | 13.6 kg (30 lb) | 14.5 kg (32 lb) |
| Voltage | 115 VAC, 50/60 Hz or 230 VAC, 50/60 Hz UK and EU Models |
115 VAC, 50/60 Hz or 230 VAC, 50/60 Hz UK and EU Models |
115 VAC, 50/60 Hz or 230 VAC, 50/60 Hz UK and EU Models |
115 VAC, 50/60 Hz or 230 VAC, 50/60 Hz UK and EU Models |
* Phasing = percentage of one cycle dispensing (systole) or refilling (diastole) pump piston
Hanna Schweighöfer1 , Christoph Rummel2 , Konstantin Mayer3 and Bernhard Rosengarten1* (2014 ) Brain function in iNOS knock out or iNOS inhibited (l-NIL) mice under endotoxic shock Intensive Care Med Exp
Anna SkoczyÅ„ska,1 Anna Wojakowska,1 Dorian Nowacki,2 Åukasz Bobak,3 Barbara Turczyn,1 Beata Smyk,1 Andrzej Szuba,1 and Tadeusz Trziszka3 (2015 ) Unsaturated Fatty Acids Supplementation Reduces Blood Lead Level in Rats Biomed Res Int
Jörg W. Wegener*,1, Florian Loga*, David Stegner†, Bernhard Nieswandt†and Franz Hofmann* (2014 ) Phospholipase D1 is involved in α1-adrenergic contraction of murine vascular smooth muscle FASEB J