Perfusion Bath for Tubular Organs (PBTO)

The Perfusion Bath for Tubular Organs (PBTO) has been designed for studying intraluminal perfused tubular organs such as trachea, atrial or venous blood vessels, intestines and vas deferens. Individual solutions can be used for intraluminal perfusion and extraluminal superfusion. Maximum tissue length is 55 mm.

Features & Benefits
Applications
Included and Required Components
Operations & Setup
 

Features & Benefits

• Accommodates individual solutions for intraluminal perfusions and extraluminal superfusion
• Controlled perfusion pressure

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Applications

• Intraluminal perfusion of tubular organs (trachea, blood vessels, intestines)
• Testing circular-action musculature, vascular tone and stents in isolated vessels

Included and Required Components

Included items: Plexiglass chassis, organ chamber with adjustable organ holder with connection cannulas, preheating coils for extraluminal and intraluminal perfusates, four different interchangeable cannulae with diameters of 1.5, 2.5, 3 and 4 mm. For smaller vessels customized stainless steel cannulae can be made on request. For micro-vessels, glass capillary pipettes pulled to the required diameter can be connected to the adapter cannulae using a silicone tube collar.

Additional equipment required: Thermocirculator, reservoir, peristaltic pumps, transducer, monitoring system setup using the PLUGSYS Amplifier System. Recording and evaluation of the signals uses BDAS or LabChart software.

Note: Two peristaltic pumps are required for the PBTO, one for intraluminal constant flow and a second for extraluminal superfusion.

Operation & Setup

Two peristaltic pumps are required, one for intraluminal constant flow perfusion and the second for extraluminal superfusion. The intraluminal perfusion pressure is generated by an adjustable afterload control system. A differential pressure transducer is used to measure the intraluminal pressure difference at the proximal and distal end of the organ.

The tissue bath is a jacketed Plexiglas bath. The holder for the cannulae can be removed from the main bath for the cannulation of the segment of tubular organs. The cannulae are fixed on sliding holders to adjust to organs of different lengths, up to 50 mm. The intraluminal pressure is controlled by one of two afterload systems available: one for low pressure applications (0 to 30 mmHg) and the second for high pressure applications (0 to 300 mmHg).