pH Control Option for IPL-1 and IPL-2

This option controls and regulates the pH in the buffer solution by carefully bubbling it with CO₂. Delivery of CO₂ maintains pH when the system is not deoxygenated with a N₂/CO₂ gas mixture.

The pH is controlled by a feedback system which regulates CO₂ gas bubbling into a dedicated small volume reservoir for minimizing foaming of perfusate. The pH is measured with a pH sensor. The pH control module (pHCM) allows setting to a desired pH, e.g. to 7.4.

A certain amount of CO₂ is necessary to hold the pH constant in a physiological range. For example, in order to keep the pH in a buffer solution at 7.4 (e.g. in a Krebs-Henseleit buffer), 5% CO₂ is necessary. If the measured pH is above this value (e.g. 7.4), a small valve opens so that CO₂ from a tank goes to a frit in the reservoir. The CO₂ changes the pH to a lower value. As soon the pH of 7.4 is reached the CO₂ valve is closed. This acts in a closed loop and regulates the pH by bubbling with CO₂. This is only necessary for perfusates which are CO₂-dependent buffered.

Note: Available by special order for IPL-4. Please inquire.

Features & Benefits

• Minimizes foaming of perfusates due to minimal gassing of 100% gas to maintain pH

Included Items

* With overflow to maintain constant perfusion solution level.

PLUGSYS modules use a total of 4 slot units. Magnetic stirrer and stirbar sold separately.