IH5-BI Biventricular Working Heart System for Rat, Guinea Pig and Rabbit

The IH-5 Langendorff system can be upgraded to the Biventricular Working Heart System (IH5-BI). For use with rat, guinea pig and rabbit hearts, it is a valuable tool for the researcher who wants tostudy whole heart cardiac function.

The complete system contains all the primary equipment you need to accomplish the basic biventricular working heart experiment, only requiring the addition of species-specific cannulae. Many options are available for the IH5-BI system to extend its capabilities. 

Advanced System Design
Features & Benefits
Applications
Measured Signals & Calculated Parameters
Included items
Species-Specific Options
Specialized Applications & Upgrades

Advanced System Design

The IH5-BI System utilizes patented flow resistance and compliance chambers to faithfully mimic the in vivo cardiac preloads and afterloads for both normal and diseased states in medium to large rodent models. The exclusive features of the system create a physiological isolated heart environment that closely resembles the actual resistance of the peripheral vasculature while allowing the entire heart to work as it does in vivo.This IH5-BI allows for in-depth ex vivo studies of the effects of pulmonary artery hypertension (PAH), hypertrophy (RVH), COPD, ARCV, emphysema and a variety of other diseases characterized by pulmonary vascular dysfunctionand right heart pathophysiology. It allows measurement of a wide range of cardiovascular parameters with unsurpassed fidelity, such as single ECG Lead II and single epicardial monophasic action potential (MAP). Integration of high fidelity pressure-volume catheters for right and left ventricle pressure-volume loop measurement is also available. 

Learn about the differences between Working (Ejecting) Heart and Biventricular Working Heart.

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Features & Benefits

• Combines the advantages of an isolated organ preparation with in situ-like perfusion features
• Allows assessment of external heart work under adjustable load for both right and left heart
• Allows a more comprehensive monitoring of functional parameters, the calculation of the external heart work and mechanical efficiency, and the highest sensitivity for various experimental manipulation in ex vivo isolated heart preparations
• Allows rapid and easy switching between biventricular working heart, left working heart and Langendorff modes
• All of the same features and benefits of the IH-5—compact, no high water columns, low resistance laminar flow paths and low system volume

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Applications

• All applications for the standard IH-5 Working Heart System
• Calcium homeostasis
• Drug effects on right heart electrophysiology and contractility 
• Ischemia/reperfusion effects on right heart
• Effects of right ventricular hypertrophy on cardiac function

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Measured Signals and Calculated Parameters

The following signals can be recorded:

• Left atrial preload pressure (left ventricular filling pressure)
• Right atrial preload pressure (right ventricular filling pressure)
• Afterload pressure (aortic pressure for calculation of systolic and diastolic pressure)
• Right ventricular afterload (pulmonary artery pressure)
• Left ventricular pressure or pressure volume loops
• Right ventricular pressure or pressure volume loops
• Cardiac output (left atrial flow), aortic, right atrial flow
• Coronary flow (subtraction of aortic flow from CO or by subtraction of right atrial flow from pulmonary artery flow)

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Included Items

The IH5-BI Base Unit (73-4238) includes:

Plexiglass stand, aorta link unit with Windkessel, adjustable artificial flow resistance for adjusting the aortic afterload pressure (with pressure gauge), preheating coil for Langendorff mode, holder for pressure transducers (aortic/perfusion pressure), tubing for in situ preparation with adapter. Also included are a left atrium cannulating system with preload reservoir and preheating coil, movable atrium connection adapter and holder for P75 (preload pressure),a column for right atrium cannulation, movable right atrium connection adapter, holder for P75 (right atrium pressure), and a right ventricle afterload system (simulates lung resistance). All cannulae must be ordered separately and depends on your animal model.

Peristaltic Pumps included are special packages suited for use with the IH-5:

73-0115 includes:

MC Pump Drive (73-3029), SB Pump Head (73-3040), 2 V Tube Bed Set (73-3045)

73-0116 includes:

MC Pump Drive (73-3026), SB Pump Head (73-3040), 2 V Tube Bed Set (73-3045)

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Required Species-Specific Additions

For a functional unit the IH5-BI Core Systems require one of these species-specific cannula sets:

Rat/Guinea Pig Biventricular Cannula Set (73-4450) includes:

• Aortic Cannula Set for rat/guinea pig hearts, 2.5 mm OD and 3.0 mm OD
• Left Atrium Cannula, 2.3 mm OD
• Right Atrium Cannula, 2.3 mm OD
• Pulmonary Artery Cannula Set, 2.5 mm OD plus Mini-Ball Joint Mounting Kit

Rabbit Biventricular Cannula Set (73-4451) includes:

• Aortic Cannula for rabbit hearts, 3.0, 4.0 and 5.0 mm OD
• Left Atrium Cannula, 2.3 mm OD
• Right Atrium Cannula, 2.3 mm OD
• Pulmonary Artery Cannula Set, 2.5 mm OD plus Mini-Ball Joint Mounting Kit

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Specialized Applications & Upgrades

All options compatible with the IH-5 Working Heart System are compatible with the IH5-BI Biventricular Working HeartSystem, excluding Multi-ECG and Multi-MAP. 

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